Added 20 μl of 10% SDS (w/v) and 20 μl of proteinase K into the tube and incubated at 55°C for 30 minutes. The lysate was cooled down and extracted once with equal volume of phenol: chloroform solution (v/v, 1:1) at 10,000rpm for 5 minutes.
Bygevoeg 20 μl van 10% SDS (w / v) en 20 μl van proteïnase K in die buis en geïnkubeer by 55 ° C vir 30 minute. Die lysate is afgekoel en keer onttrek met gelyke volume van fenol: Chloroform Oplossing (v / v, 1: 1) by 10,000rpm vir 5 minute.