Eluting at 27 min was compound 3 (8

Eluting at 27 min was compound 3 (8.9 mg), well separated from
other peaks (chromatogram in Supporting Information). At 25 min,
the first of a poorly resolved set of three compounds was further
purified by rechromatography in the same solvent without the 100%
CH3OH step in the gradient to give 4.3 mg of 4. The middle peak was
rechromatographed on a 10 × 250 mm C8 Dynamax column, isocratic
at 80% aqueous MeCN for 25 min, to 100% MeCN at 30 min to give
4.8 mg of 5, eluting at 16 min. The earliest cluster of peaks in the
primary HPLC separation yielded 6 (3.6 mg, 10 min), 7 (3.5 mg, 12
min), 8 (3.8 mg, 8 min), and 9 (4.0 mg, 9 min).

ผลลัพธ์ (แอฟริกา) 1:

Verwyderende op 27 min was Saamgestelde 3 (8,9 mg), goed Geskei van
Ander Peaks (chromatogram in Ondersteunende inligting). Op 25 min,
was die eerste van 'n swak besluit SET Drie van verbindings verder
gesuiwer deur Rechromatography in dieselfde Sonder die Solvent 100%
CH3OH Stap in die helling tot 4.3 mg 4. Die MIDDLE Peak was gee
op 'n 10 × 250 mm Rechromatographed. Dynamax C8 kolom isocratic
teen 80% waterige MeCN vir 25 min, tot 100% MeCN op 30 min om te gee
4,8 mg van 5, verwyderende op 16 min. Vroegste Cluster van die pieke in die
primêre HPLC Skeiding opgelewer 6 (3,6 mg, 10 min), 7 (3,5 mg, 12
min), 8 (3,8 mg, 8 min), en 9 (4,0 mg, 9 min).