Chemical AnalysesSamples of feed we

Chemical Analyses
Samples of feed were analyzed for DM, N, Ca, P,
and AA according to AOAC (2000) methods. Amino
acids were determined by hydrolyzing samples with 6
mol/L HCl for 24 h at 110°C (AOAC, 2007) and analyzed
using an Amino Acid Analyzer (Hitachi L-8800;
Hitachi, Ltd., Tokyo, Japan). Methionine and cysteine
were determined as methionine sulfone and cysteic acidafter cold performic acid oxidation overnight and hydrolyzed
with 7.5 mol/L HCl for 24 h at 110°C using
an amino acid analyzer (Hitachi L-8800; Hitachi, Ltd.).
Tryptophan was determined after LiOH hydrolysis for
22 h at 110°C using HPLC (Agilent 1200 Series; Agilent
Technologies Inc., Santa Clara, CA). The GE concentration
was measured using an adiabatic bomb calorimeter
(Parr 6300 Calorimeter; Parr Instrument Company,
Moline, IL). Samples of feces were analyzed for DM,
N, and GE. Samples of urine were analyzed for N using
fresh material. The concentration of GE in urine samples
was measured after 4 mL of sample were dripped into
2 filter papers in a special crucible and dried for 8 h at
65°C in a drying oven, and then the energy content was
determined using an adiabatic bomb calorimeter.

ผลลัพธ์ (อังกฤษ) 2:

Chemical Analyses
of Feed Samples were analyzed for DM, N, Ca, P,.
and AA according to AOAC (the 2,000th) methods. Amino
acids were Samples Determined by hydrolyzing with 6.
mol / L HCl for 24 h at 110 ° C (AOAC, 2,007th) and analyzed.
using an Amino Acid Analyzer (Hitachi L-8800;
Hitachi, Ltd., Tokyo, Japan). Methionine and cysteine
​​were Determined As methionine sulfone and Cysteic Acidafter cold Performic acid oxidation Overnight and Hydrolyzed.
with 7.5 mol / L HCl for 24 h at 110 ° C using.
an amino acid Analyzer (Hitachi L-8800; Hitachi, Ltd.).
Tryptophan was Determined After LiOH hydrolysis for
22 h at 110 ° C using HPLC (Agilent Series 1200th; Agilent.
Technologies Inc., Santa Clara, CA). The GE Concentration
was measured using an adiabatic Bomb Calorimeter.
(Parr 6300 Calorimeter; Parr Instrument Company,.
Moline, IL). Samples of feces were analyzed for DM,.
N, and GE. Samples of urine were analyzed for N using.
Fresh material. The Concentration of GE in urine Samples.
was measured After 4 mL of Sample were dripped Into.
2 Filter papers in a special Crucible and Dried for 8 h at.
65 ° C in a Drying Oven, and then The Energy content was.
Determined using an adiabatic Bomb Calorimeter .