Diagnosis by PCR method is not acceptable because the display of the control bar's DNA is difficult. Cause the result. So have developed a new method PCR based Real-Time PCR is a method which is a molecular detection methods can be better and more quickly, and be able to distinguish the small molecules, although the same way by the Real-Time PCR analysis of curvature relies on graph (Pewpan M. Intapan et al., 2009), the result is a much more credible. This is used to select the various diagnostic methods depends on the objectives of each study.
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