So we made a new design primer. • Use NCBI's genbank sequence for each whole grass that we want to design primer. • Using the program primer3 primer design program in order to apply the primer by the program design (for both forward and reverse primer primer) goes against the whole sequence of each type of grass that we wanted to try to see this grass 5 conserve the same region or position the base, which is about 18-23 base pair by trial, we will put the whole sequence of each type of grass that we want to go down in Microsoft word, and then go Home and Find the program Navigator leading primer3 primer design (both forward and reverse primer primer) to compare and see if there is a position of the same region or conserve the base. In what kind of grass.• Using the program Analyzer primer Oligo in a quality check with the program primer3 primer design (both forward and reverse primer primer) to check. A good quality primer has a property as follows:
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