3.2.3 preparation solution for E.coli bacteria in killing on the workpiece. 1. raising E.coli bacteria in liquid volume measurement in a bottle size TSB 250 ml volumetric adjustment by TSB weighed 23.5 grams with distilled water. 2. prepare a slurry 1/500 volumetric measurements in a bottle size, NB 1000 ml peptone beef extract weighing 0.1 0.03 grams, G 0.01 g, sodium chloride, and adjust the volume with distilled water. 3. prepare a Phosphate buffer solution slurry preparation (for fuel consumption). 2000 ml volumetric measuring by bottle, weighing 17.0 g sodium chloride compounds and Phosphate buffer extraction solution Stock solution 2.5 ml from adjusting the volume with distilled water, insert each tube test tube shot 9 ml tube, 200 count. 4. prepare Plate count agar fuel meal (PCA) PCA fuel meal weighing by 47.0 g adjust volume 2-liter with distilled water and heat, stirring with solvents. 5. bring a solution in 1-ml volume 9 and solvents 2.1 ml volume in the mix into the test tube. Measured with a Spectrophotometer to OD and record results3.2.4 test the ability to kill the bacteria E.coli on the target 2% TiO2/ABS.1. extraction solvents infection with a Spectrophotometer to OD measurements. 2. extraction solvents in 0.4 ml drops ometer 1 11. Ml bottles Rennes there are very view put in distilled water are. 99 ml drops down on the work piece (for the test.Onto the workpiece by placing the workpiece, test, kill the bacteria onto a Petri dish. UV light leads to 2 hours 3. UV projection two hours already. Use tweezers, dip, alcohol 95% light stew, cubed pieces. 4. fill water into a Sterile 10 ml DI Petri plate. 5. extraction solvents in a Petri plate 4.10 ml test tubes put in Phosphate buffer solution containing solvents (for fuel consumption) dilute solution concentration in vitro from 10-1 to 10-5 fuel good dispersion. 6. extraction solvents dilute in vitro 1.0 ml agar petri plate by inserting the meal down Petri plate PCA fuel first. Wait, jelly cabinets, solid foods into curing at a temperature of 35-37° C time is 24 hours. 7. count and record results
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