The preparation process of the CMTAB is shown in Scheme 1. In the carboxyl group activation stage the carboxyl, group reacts. With SOCl2 a reagent, widely used in changing the carboxyl into highly reactive acyl chloride [,]. The 28 29 molar ratio of. SOCl2 to EDTA was fixed as 1: 1 to ensure only one carboxyl group in the EDTA can be acyl chlorinated. Then the acyl chlorinated. EDTA can be grafted to the OTAB matrix through two routes. In Route 1 the acyl, chloride group reacts with the hydroxyl. And amino groups on the surface of the biomass and is grafted. In, route 2 due to the high reaction.Activity the acyl, chloride can also react with the other carboxyl in EDTA to form an internal carboxylic anhydride group. Which may further react with the hydroxyl and amino groups so as to be grafted to OTAB.
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