We describe a new high performance

We describe a new high performance liquid chromatography coupled with ultraviolet detection method
for the quantification of plasma concentration of oral iron chelating agent deferasirox. A simple protein
precipitation extraction procedure was applied on 500 l of plasma aliquots. Chromatographic separation
was achieved on a C18 reverse phase column and eluate was monitored at 295 nm, with 8 min of analytical
run. This method has been validated following Food and Drug Administration procedures: mean intra and
inter day variability was 4.64 and 10.55%; mean accuracy was 6.27%; mean extraction recovery 91.66%.
Calibration curves ranged from 0.078125 to 40 g/ml. Limit of quantification was set at 0.15625 while
limit of detection at 0.078125 g/ml. We applied methodology developed on plasma samples of thalassaemic patients treated with deferasirox, finding correlation between deferasirox plasma concentrations
and serum ferritin levels. This methodology allowed a specific, sensitive and reliable determination of
deferasirox, that could be useful to perform its therapeutic monitoring and pharmacokinetic studies in
patients plasma.

ผลลัพธ์ (อังกฤษ) 2:

We describe A New HIGH Performance Liquid chromatography coupled with Ultraviolet Detection Method.
for The quantification of Plasma Concentration of oral Deferasirox Iron chelating Agent. A Simple protein
extraction procedure was Precipitation Applied on 500? L of Plasma aliquots. Chromatographic Separation
was achieved on C18 Reverse Phase A and Column eluate was monitored at 295 NM, with 8 min of Analytical.
Run. This Method Food and Drug Administration has been Validated Following procedures: intra and mean.
Inter Day variability was 4.64 and 10:55%; mean Accuracy was 27.6%; mean extraction Recovery 91.66%.
Calibration Curves ranged from .078125 to 40? G / ml. Limit of quantification was Set at .15625 while.
Limit of Detection at .078125? G / ml. We developed methodology on Applied Plasma Samples of Thalassaemic patients treated with Deferasirox, Finding correlation between Deferasirox Plasma concentrations.
and Serum ferritin levels. This methodology allowed A Specific, sensitive and reliable Determination of.
Deferasirox, that could be Useful to Perform its pharmacokinetic and Studies in Therapeutic Monitoring.
patients Plasma.