รูปที่ 5c แสดงให้เห็นถึง Decolorization time-course of synthetic dyes LG , by immobilized or free laccase in the presence and absence of redox mediator (HBT, 1 mM). พบว่า In the presence of HBT (1 mM) the immobilized enzyme exhibited efficient LG decolorization (86.5%) after 24 h.