2.5. Analysis of SSF samples by HPLC
Samples were analyzed for cellobiose glucose xylose xylitol,,,,,,, glycerol
succinic acid Acetic acid and ethanol, by HPLC. Twenty
microliters of each sample were analyzed by HPLC with a refractive
index detector. (RID) (Agilent 1100 Santa Clara, CA), using an Aminex
HPX-87H organic acid column (,,) Biorad Hercules CA at 60 C.
The mobile. Phase was 0.01 N H2SO4 pumped at 0.6 mL / min for
30 min.
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