TLC Analysis of the Extracts of Mangosteen Fruit
Rinds from 13 Locations
TLC fingerprints were performed on a precoated
aluminium plate of silica gel 60F254 (10×10 cm) using
chloroform : ethyl acetate : methanol (80:10:5) as a
mobile phase. The developing distance was 8.0 cm.
After removing the plate from the chamber, the plate
was dried using an air dryer and sprayed with 10 %
sulfuric acid in ethanol, followed by heating at 110°C for
10 min. The plate was examined under ultraviolet light
(366 nm). The hRf value of main component was
determined comparing with the hRf value of α-
mangostin reference standard. Video densitometry of
the chromatoplate was carried out using CAMAG
Reprostar 3 with cabinet cover and mounted digital
camera