Fresh leaves were processed with a, Clevenger apparatus and the EO was obtained by hydrodistillation protected against,, Light, and heat and stored until behavioral assays. Previously the EO, was analyzed by gas chromatography coupled with mass. Spectrometry (GC / MS) as described (Blanco et al, 2009).
Thin layer chromatography (TLC) of EO and decoction was performed. On Silica gel 60 (250, MFresh leaves were processed with a, Clevenger apparatus and the EO was obtained by hydrodistillation protected against,, Light, and heat and stored until behavioral assays. Previously the EO, was analyzed by gas chromatography coupled with mass. Spectrometry (GC / MS) as described (Blanco et al, 2009).
Thin layer chromatography (TLC) of EO and decoction was performed. On Silica gel 60 (250, M10 cm *, 20 cm Sigma - Aldrich apertium
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