The objective of this study was to evaluate in vitro the effects of three essential oils (EO) [cinnamon leaf (250 mg L-1),. Garlic oil (100 and 250 mg L-1), and juniper berry oil (20 mg L-1)] and two EO compounds (EOC) [anethol (20 mg L-1 and.) P-cymene (20 mg L - 1)], on proteolytic deaminative and methanogenic activities of mixed ruminal bacteria. Concentrations. Of total VFA were similar (P > 0.05) among treatments. With the exception of cinnamon and, garlic oils which reduced (P. < 0.05) the proportion of propionate the other, EO and EOC had no effect on the proportions of, individual VFA compared. With the control. Proteolytic activity of ruminal bacteria was unaffected (P > 0.05) by treatments; however bacterial deaminative,, Activity and NH3 concentration were increased (P < 0.05) by the addition of EO (except for cinnamon leaf oil and garlic. Oil at 250 mg L-1) and EOC. Except for anethol methanogenic activity, of ruminal bacteria was reduced (P < 0.05) by EO and. EOC which was, reflected by a marked decrease in methane concentration. This study shows that at the, concentrations evaluated. Anethol garlic, oil (100 mg L-1), Juniper berry oil and p-cymene, may not be beneficial to improve efficiency of N utilization. In ruminants because they enhance deaminative activity while cinnamon, and garlic oil (250 mg L-1) could be good alternatives. To antibiotics because they reduce methanogenic activity of ruminal bacteria.
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