Indirect ELISA procedure done with a worm hole, coating the bottom 160 maikhrokram concentration per ml. Serum dilution buffer with blocking code (1% BSA in PBS) in a ratio of 1:100 is a primary antibody.
Step indirect ELISA coating antigen hole with a concentration of 160 micrograms per milliliter. Diluted serum of cattle with blocking buffer (1% BSA in PBS) at a ratio of 1: 100 as the primary antibody.