All chemicals were obtained from Sigma-Aldrich.Laboratory (St. Louis MO USA) unless otherwise,,,Stated. Oocytes from Nelore and Holstein cows were.Divided into two treatment groups. In, group 1 oocytes.Of both breeds were aspirated 4 h after acquisition of.Ovaries in the abattoir whereas in, group 2 oocytes of,,Both breeds were aspirated 6.5 h after ovaries were.Acquired (the interval from death to ovary acquisition.Was approximately 30 min). Nelore oocytes were fertilized.With frozen-thawed semen from, Nelore bullsWhereas Holstein oocytes were fertilized with semen.From Gir and Holstein bulls. Six bulls from each of the.Three breeds were used.
การแปล กรุณารอสักครู่..