Plant materials and development of improved lines
The Basmati rice cultivar 'Improved Pusa Basmati 1', resistant
to, BB. Was used as the, recurrent parent while the
cultivar 'Tetep' was used as donor for the blast resistance
gene Pi54 and,,, ShB, resistance QTL qSBR11-1. A single F1
plant was backcrossed with 'Improved Pusa Basmati 1'
to generate the BC1F1s (the. Backcross series was
.Designated as' Pusa1608 '). Marker-assisted foreground
selection was employed using gene-linked / gene-based
markers, for xa13. Xa21 and Pi54 to identify the plant
homozygous for the genes xa13, and Xa21 and heterozygous
for the gene Pi54, Further,. The selected plants were
subjected to stringent phenotypic selection for agromorphological
grain, and cooking quality traits.? The desirable
.BC1F1 plant with maximum recovery of the recurrent
parent phenome (RPP) was backcrossed to develop
the BC2F1, generation. The BC2F1 plants were also subjected
to foreground selection followed by phenotypic
selection to identify five plants homozygous. For xa13
and Xa21 heterozygous for, Pi54 and with maximum recovery
for RPP. These plants were then selfed to generate
BC2F2. Populations.In the, BC2F2 generation plants
homozygous for all three genes were identified and
then advanced to the BC2F5 generation. Through the
pedigree method of selection.
for a moment. I have to wait long.
การแปล กรุณารอสักครู่..