Immunohistochemistry (IHC)Microscop

Immunohistochemistry (IHC)
Microscope image of rabies virus-infected neuronal cell
This slide shows a rabies-infected neuronal cell with intracytoplasmic inclusions. The red stain indicates areas of rabies viral antigen by using IHC or avidin-biotin complex (ABC) technique.
IHC methods for rabies detection provide sensitive and specific means to detect rabies in formalin-fixed tissues. These methods are more sensitive than histologic staining methods, such as H&E and Sellers stains. Like the dFA test, these procedures use specific antibodies to detect rabies virus inclusions. The techniques use enzyme-labeling systems that increase sensitivity. In addition, monoclonal antibodies may be used to detect rabies virus variants.
The presence of Negri bodies is variable. Histologic staining for Negri bodies is neither as sensitive nor as specific as other tests. Some experimentally-infected cases of rabies display Negri bodies in brain tissue; others do not. Histologic examination of tissues from clinically rabid animals show Negri bodies in about 50% of the samples; in contrast, the dFA test shows rabies antigen in nearly 100% of the samples. In other cases, non-rabid tissues have shown inclusions indistinquishable from Negri bodies. Because of these problems, the presence of Negri bodies should not be considered diagnostic for rabies.

ผลลัพธ์ (มาเลย์) 1:

Immunohistochemistry (IHC)
Microscope Image of RABIES Virus-Infected Cell neuronal.
This slide Shows A RABIES-Infected Cell with intracytoplasmic neuronal inclusions. The Red stain RABIES Indicates Areas of Viral Antigen by using avidin-biotin or IHC Complex (ABC) Technique.
IHC Detection methods for RABIES provide sensitive means to detect and Specific RABIES in formalin-Fixed tissues. These methods are more sensitive than histologic staining methods, such as H & E and Sellers stains. Like the dFA test, these procedures use specific antibodies to detect rabies virus inclusions. The techniques use enzyme-labeling systems that increase sensitivity. In addition, monoclonal antibodies may be used to detect RABIES Virus variants.
The Presence of Negri Bodies is Variable. Histologic staining for Negri bodies is neither as sensitive nor as specific as other tests. Some experimentally-infected cases of rabies display Negri bodies in brain tissue; others do not. Histologic examination of tissues from clinically rabid animals show Negri bodies in about 50% of the samples; in contrast, the dFA test shows rabies antigen in nearly 100% of the samples. In other cases, non-rabid tissues have shown inclusions indistinquishable from Negri bodies. Because of these problems, the presence of Negri bodies should not be considered diagnostic for rabies.