3.Freeze -drying: When freeze-dryin

3.Freeze -drying: When freeze-drying microorganisms in vials or ampoules without cotton plugs or other bacteriological filters, the microorganisms can be carried from the container and contaminate the freeze-drying system . Microbial contamination can be found on the outside of the vial or ampoule and on parts of the freeze -drying system such as the condenser. A system should be designed to monitor the contamination level, and decontamination procedures should be implemen ted if necessary. Take care to properly treat freeze -d riedcul tures prior to disposal. To autoclave freeze-dried cultures, open the vial or ampoule to allow penetration of the steam . An alternative to autoclaving is to heat the preparations in a hot air oven at 180 °C for 4 h.

3.Freeze -drying: When freeze-drying microorganisms in vials or ampoules without cotton plugs or other bacteriological filters, the microorganisms can be carried from the container and contaminate the freeze-drying system. Microbial contamination can be found on the outside of the vial or ampoule and on parts of the freeze -drying system such as the condenser. A system should be designed to monitor the contamination level, and decontamination procedures should be implemen ted if necessary. Take care to properly treat freeze -d riedcultures prior to disposal. To autoclave freeze-dried cultures, open the vial or ampoule to allow penetration of the steam. An alternative to autoclaving is to heat the preparations in a hot air oven at 180 ° C for 4 h.

3.Freeze - drying: When freeze-drying microorganisms in vials or ampoules without cotton plugs or other bacteriological. Filters the microorganisms, can be carried from the container and contaminate the freeze-drying system. Microbial contamination. Can be found on the outside of the vial or ampoule and on parts of the freeze - drying system such as the condenser. A system. Should be designed to monitor the contamination level and decontamination, procedures should be implemen) ted if necessary.? Take care to properly treat freeze - D riedcul) tures prior to disposal. To autoclave, freeze-dried cultures open the vial. Or ampoule to allow penetration of the steam. An alternative to autoclaving is to heat the preparations in a hot air oven. At 180 ° C for 4 h.