In the present investigation we looked, into the relationship.Between the genetic diversity of microbial populations.In compost and the enzymatic activity of chitinase. The.Suppressive effects on different plant pathogens by composts.Are well documented (Hoitink et al, 2001) but in.Most cases the actual mechanisms by which this happens.Are unknown. However it has, been suggested that chitinase.Activity may play a role by causing lysis of pathogenic.Fungi (Boulter et al, 2000; Labrie et al. 2001). Therefore,,We tried to induce chitinase activity by supplementing.Two types of compost (source separated organic household.Waste and garden / Park waste compost) with chitin and.Measured the chitinase activity using a very sensitive fluorogenic.Substrate that previously has been used to detect.Enzyme activity in soils (e.g, Marx et al, 2001). To link.The chitinase activity of the microbial populations in the.Compost to the genetic diversity we employed, denaturing.Gradient gel electrophoresis (DGGE). Because chitin.Has high nitrogen content (6.8% by weight) and as a.
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