2. Research Method.2.1. Place and Time of Research.The research was conducted at the Laboratory of Agricultural Technology Faculty of, Agriculture UNSOED. The.Research held from March until July 2015.2.2. Materials.Materials needed research consisting of: Nicolaia speciosa flower. Chemicals used among other technical.Solvents namely ethanol, 96%; encapsulant (gelatin and cyclodextrin), ethanol 99.8% (PA), linoleic acid distilled,,,, water ammonium thiocyanate 0.02 M FeCl2 tetrahydrate concentrated, gas, HCl N2. Other materials needed.Include a coarse, filter paper Whatman No. 41 paper plastic paper label,,, towels paper and aluminum foil.2.3. Equipment.The tools used in this research that cabinet dryer dryers blenders analytical scales rotary,,, evaporator (Bibby.RE 200), shaker oven (,, Memmert Japan), desiccators pipettes (,, Pyrex Germany), pH meter. Tool for the analysis of.Total phenol and antioxidant activity consists of spectrophotometers centrifuges glass tools (,,,, Pyrex Germany),, vortex Fial tubes micropipette (Gilson), and bath tobacco leaf waste ie shaker,,, rotary tube N2. Tool to extract.Formulation a set, of tools for testing antimicrobial activity glasses vortex micropipettes incubator 37o,,,,C.Desiccator refrigerator and, vacuum pump.
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