After infection of clostridium positive streak came down in TSC agar with egg yolk emulsion by pouring the egg yolk agar TSC no emulsion curing in oxygen conditions. Temperature 35 24 hours.
After the positive infections of clostridium streak coming into the TSC agar containing egg yolk emulsion with TSC agar pour over the egg yolk emulsion is incubated in the absence of oxygen at a temperature of 35 to 24 hours.
After the positive infections of Clostridium come streak into TSC agar with Egg Yolk Emulsion poured TSC agar at. Egg Yolk Emulsion curing condition without oxygen, temperature, time 35 24 hours.