3.3. Antioxidant activities Antioxi

3.3. Antioxidant activities
Antioxidant activities as measured by DPPH, FRAP, and ORAC methods are shown in Table 3. DPPH radical is a stable free radical in a group of reactive nitrogen species. The DPPH assay is based on the ability of antioxidants to reduce the DPPH radical resulting in colour change from purple/blue to yellow (Prior, Wu, & Schaich,2005). The DPPH value of the three fruits ranged widely from 61 lmolTE/g in A. velutinosum Blume to 489 lmolTE/g in P. emblica L. The antioxidant activities of P. emblica L. (302–592 lmolTE/g) and A. velutinosum Blume (9–94 lmolTE/g) was widely distributed
due to the maturity of the fruits and growing locations/conditions (Ferreyra et al., 2007; Han et al., 2008).
FRAP measured the ability of antioxidants to reduce the ferric-tripyridyltriazine (Fe3+-TPTZ) complex to the blue-colour complex of ferrous-tripyridyltriazine (Fe2+-TPTZ) in acidic conditions (Benzie & Strain, 1996). P. emblica L. had the highest FRAP value (652 ± 260 lmolTE/g), followed by S. pinnata (L.f.) Kurz (424 ± 39 lmolTE/g) and A. velutinosum Blume (118 ± 81 lmolTE/g).
ORAC assay is one common method for evaluating antioxidant capacity. Its principle is similar to the biological function of chain-breaking antioxidants (Ou et al., 2002). The assay is based upon the reaction between the peroxyl radical and a fluorescent probe. The radical oxidises the probe resulting in a decrease in fluorescence intensity. In the presence of antioxidants, the reaction is extended (Prior et al., 2005). A similar trend in antioxidant activ-ities for the three fruits was also observed by the ORAC assay com-pared to DPPH and FRAP assays, wherein the ORAC activity of P.emblica L. > S. pinnata (L.f.) Kurz > A. velutinosum Blume. A strong correlation between total phenolic content and DPPH, FRAP, and
ORAC values was observed in this study (r = 0.94, 0.94 and 0.89,respectively). This relationship has been reported in many other studies (Han et al., 2008; Maisuthisakul et al., 2007), which con-firmed that phenolics were the major contributor to antioxidant activity.

ผลลัพธ์ (อังกฤษ) 2:

3,3. Antioxidant activities
Antioxidant activities measured by As DPPH, FRAP, and ORAC methods are shown in Table 3. DPPH Radical is a free Stable Radical in a Group of reactive Nitrogen Species. The DPPH assay is based on the ability of antioxidants to reduce the DPPH radical resulting in colour change from purple / blue to yellow (Prior, Wu, & Schaich, 2005). The DPPH value of the three fruits ranged widely from 61 lmolTE / g in A. velutinosum Blume to 489 lmolTE / g in P. emblica L. The antioxidant activities of P. emblica L. (302-592 lmolTE / g) and A. Velutinosum Blume (9-94 LmolTE / G) was widely Distributed.
Due to The Maturity of The Growing Fruits and Locations / conditions (Ferreyra et AL., 2,007; Han et AL., two thousand and eight).
FRAP measured The Ability of antioxidants to Reduce The. ferric-tripyridyltriazine (Fe3 + -TPTZ) complex to the blue-colour complex of ferrous-tripyridyltriazine (Fe2 + -TPTZ) in acidic conditions (Benzie & Strain, 1996). P. emblica L. had the highest FRAP value (652 ± 260 lmolTE / g), followed by S. pinnata (Lf) Kurz (424 ± 39 lmolTE / g) and A. velutinosum Blume (118 ± 81 lmolTE / g).
ORAC assay is one common method for evaluating antioxidant capacity. Its principle is similar to the biological function of chain-breaking antioxidants (Ou et al., 2002). The assay is based upon the reaction between the peroxyl radical and a fl uorescent probe. The radical oxidises the probe resulting in a decrease in fl uorescence intensity. In the presence of antioxidants, the reaction is extended (Prior et al., 2005). A similar trend in antioxidant activ-ities for the three fruits was also observed by the ORAC assay com-pared to DPPH and FRAP assays, wherein the ORAC activity of P.emblica L.> S. pinnata (Lf) Kurz> A. velutinosum. Blume. A strong correlation between total Phenolic content and DPPH, FRAP, and.
ORAC values ​​was observed in this Study (R = 0.94, 0.94 and 0.89, respectively). This relationship has been reported in many other studies (Han et al., 2008; Maisuthisakul et al., 2007), which con- fi rmed that phenolics were the major contributor to antioxidant activity.

ผลลัพธ์ (อังกฤษ) 3:

3.3. Antioxidant activities
Antioxidant activities as measured, by DPPH FRAP and ORAC, methods are shown in Table 3. DPPH. Radical is a stable free radical in a group of reactive nitrogen species. The DPPH assay is based on the ability of antioxidants. To reduce the DPPH radical resulting in colour change from Purple / blue to yellow (,,, Prior Wu & Schaich 2005).The DPPH value of the three fruits ranged widely from 61 lmolTE / g in A. Velutinosum Blume to 489 lmolTE / g in P. Emblica. L. The antioxidant activities of P. Emblica L. (302 - 592 lmolTE / g) and A. Velutinosum Blume (9 - 94 lmolTE / g) was widely distributed
due. To the maturity of the fruits and growing locations / conditions (Ferreyra et al, 2007; Han et al, 2008).
.FRAP measured the ability of antioxidants to reduce the ferric-tripyridyltriazine (Fe3 - TPTZ) complex to the blue-colour. Complex of ferrous-tripyridyltriazine (Fe2 - TPTZ) in acidic conditions (Benzie, & Strain 1996). P. Emblica L. Had the highest. FRAP value (652 edge 260 lmolTE / g), followed by S. Pinnata (L.f.) Kurz (424 edge 39 lmolTE / g) and A. Velutinosum Blume (118 edge 81 lmolTE / g).
.ORAC assay is one common method for evaluating antioxidant capacity. Its principle is similar to the biological function. Of chain-breaking antioxidants (Ou et al, 2002). The assay is based upon the reaction between the peroxyl radical and a. fl UORESCENT probe. The radical oxidises the probe resulting in a decrease in fl uorescence intensity. In the presence, of antioxidantsThe reaction is extended (Prior et al, 2005). A similar trend in antioxidant ACTIV-ITIES for the three fruits was also. Observed by the ORAC assay com-pared to DPPH and FRAP assays wherein the, ORAC activity of P.emblica L. > S. Pinnata (L.f.). Kurz > A. Velutinosum Blume. A strong correlation between total phenolic content, and DPPH FRAP and
ORAC, values was observed. In this study (r =, 0.94 0.94, and 0.89 respectively). This relationship has been reported in many other studies (Han et al, 2008; Maisuthisakul et. Al, 2007), which con - fi rmed that phenolics were the major contributor to antioxidant activity.
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