Remove the unbound ECM substrate and block the coated dishes with 3 ml of 2 mg ml - 1 bovine serum albumin for 1 h at 37 ° C.? Then wash the, dishes once with PBS and refill the dishes with 3 - 5 ml of media before plating the cells. For the particular. Cell, type used the appropriate amount of serum in the medium during the in vitro scratch assay is required to be, determined. It is recommended to use a lower percentage of serum than that used in the growth media to minimize, cell proliferation. But just sufficient to prevent apoptosis and / or cell detachment.
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