The head cold rice (Dioscorea birmanica Prain & Burkill) is a Thai herbal plants in my house, there are many reptiles Dioscoreaceae is a climbing plant trees accumulated underground restaurant in a root (rhizome) and all have a thorn (Wilkin and Thapyai,2009), popular with the ancestry of a person with other types of herbs for treatment of my aunt yellow blood poisoning cancer and AIDS (Itharat and workgroups,2010), and painted, there is a need to use the head cold rice, but it takes a long time to expand on the nature of plants and trees will be able to produce enough to (a) The mission of Craufurd,2001), and is therefore, technical, cultured plant tissues to increase forest dish in the beginning of which is currently expanding herbal plants with many kinds of tissue, such as cultivating Curcuma zedoaria (Loc and workgroups, 2005), Piper methysticum (Zhang and workgroups, 2008) ,and Dioscorea bulbifera (Narula and workgroups, 2003), but also do not have a study of cultured tissue in the cold rice (D.birmanica, so, in this research, it was the study of the type and intensity of gasoline out of the forest and the dish up to the root, and drew the cold rice (D. birmanica) in sterile condition
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