This research aims to determine the achievement in Nano-zinc oxide materials. To prevent fungal leaf disease of Marigold and the frequency in Nano-zinc oxide spray material that is suitable as a type of marigold flowers that can grow revenues, and as the flowers are important economic, Marigold is rather high price. Planting Marigold today often the disease that causes the output is quite low. Fungal leaf spot disease found in most Farmers need to use chemicals, resulting in a loss of both the cost and the environment. Nano zinc oxide materials currently used in agriculture is that interesting. One way to help farmers who cultivate Marigold has produced. In this research, Nano-zinc oxide materials come to spray them with Marigold. Nano zinc oxide materials with properties to resist bacteria and fungi and therefore should be used with Marigold. In this research, plant Marigold by converting each, divided into 4 clear conversion cost and Marigold 10 spraying the Nano zinc oxide materials as follows: 1. the conversion of a non-material, Nano-zinc oxide 2. nano-materials that are sprayed zinc oxide every day. 3. nano-materials that are sprayed zinc oxide every 3 times and 4. nano-materials that are sprayed zinc oxide every 5 days. ผลการทดลองได้พบว่าการใช้วัสดุนาโนซิงค์ออกไซด์สามารถป้องกันการเกิดโรคเชื้อราใบจุดของดาวเรือง ซึ่งผลปรากฏว่าแปลงที่ 2 ซึ่งฉีดพ่นวัสดุนาโนซิงค์ออกไซด์ทุกวันสามารถป้องกันโรคเชื้อราใบจุดของดาวเรืองได้ดีกว่าแปลงอื่นๆ และพบว่ามีการเจริญเติบโตและมีผลผลิตดีที่สุด
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