Screening of lipase producing isola

Screening of lipase producing isolate on plate
agar
Lipase activity was tested on SGC agar
medium containing 2 % of Tween 80 and
supplemented with 1, 2, 3 and 4 M NaCl, while
the control culture lacked NaCl. The bacterial
isolates were spotted onto test agar and then
incubated at 37°C for 10 days. A positive reaction
for the lipase test was indicated by the opaque zone
around the colony. The width of opaque zone was
considered to be directly related to the amount of
extracellular lipase produced. The ratio of enzyme
production and growth were defined as A/B, where
A was the diameter of opaque zone (mm) and B
was the diameter of the colony size (mm). One
isolate having the highest lipase activity was
selected and subjected to the following
experiments.
3. Identification of bacterial strain
The morphological and physiological
characteristics of the selected isolate were
performed according to the standard method
(Cowan, 1974; Krieg and Holt, 1984; Washington,
1985; Baron and Finegold, 1990; Delost, 1997).
Determination of 16S rRNA sequence was
performed by direct sequencing of enzymatically
amplified DNA with specific oligonucleotide
primer (Arahal et al., 1996). The 16S rRNA
sequence of the selected isolate was analysed by
using an automatic DNA sequence and compared
to 16S rRNA sequence with other bacteria from
GenBank of National Center for Biotechnology
Information (NCBI).

ผลลัพธ์ (อังกฤษ) 2:

Screening of lipase producing isolate Plate on
agar
agar Lipase Activity was tested on SGC
Medium containing 2% of Tween 80 and
supplemented with 1, 2, 3 and 4 M NaCl, while
the Control Culture lacked NaCl. The bacterial
isolates were then spotted onto Test agar and
incubated at 37 ° C for 10 days. A positive Reaction
Test for the lipase was indicated by the opaque Zone
Around the Colony. The width of opaque Zone was
considered to be directly related to the amount of
extracellular lipase produced. The ratio of enzyme
Production and growth were defined as A / B, where
A was opaque of the Zone Diameter (mm) and B
was the Colony of the Diameter Size (mm). One
isolate having the highest lipase Activity was
selected and subjected to the following
experiments.
3. Identification of bacterial strain
The morphological and physiological
characteristics of the selected isolate were
performed according to the standard method
(Cowan, 1,974th; Krieg and Holt, 1 984; Washington,
the 1,985th; Baron and Finegold, the 1990th; Delost, one thousand nine hundred ninety-seven).
Determination of 16S rRNA. Sequence was
performed by Direct sequencing of enzymatically
amplified DNA with specific oligonucleotide
Primer (Arahal et al., 1996). 16S rRNA the
Sequence of the selected isolate was analyzed by
using an Automatic Sequence DNA and compared
to 16S rRNA Sequence with Other bacteria from
GenBank National Center for Biotechnology of
Information (NCBI).