Research methods 1
. Morphological characteristics of a white flower
TA morphological characteristics of the crescent of white flowers the stems, roots, leaves, flowers, fruit and seed
2 tissue culture of white flowers.
.Sample preparation plants:? Choose components from the beginning with a healthy growth, no disease and insects. The Nata peak to clean clean with water several times. Then cut unwanted parts get up as early as possible the remaining approximately 3-5 cm
.Study of the excised:? The field took the top white flowers come out of solution, Clorox (Clorox 1.40 percent (V / V) sodium hypochlorite) at a concentration of 5 10 20 percent and that mixture of catch leaves the skin (Tween) 1-2 drops. Take the time to purify different. Then the eye field that sterilized and cutting it to approximately 0.5-0.8 cm, with a side only of 1 eyes. Then go to cultured on MS does not fill any growth regulators, fill the aunt sucrose 30 g per liter, and jelly 5 grams per liter. Applied Experiment 4 left Sha 10 per sample.And field cultured cultured under room temperature 25 C, light intensity, 2500 Deluxe for 15
day increasing number of peaks:? Parts of the field survival of crescent of white flowers that sterilized, cutting it to approximately 0.5-0.8 cm, with a side only of 1 eye moved to cultured on Murashige and Skoog (1962) or MS fill BAP. The paint in one tissue. After the bottle is placed on the inner developing tissue cultured under room temperature, 25Light intensity, 2500 Deluxe for 16 hours per day to observe the experiment
; Nara: you can plant a white flowers from 3.3.2 method.4 moved into formula MS fill NAA by applied in one tissue. Na bottle after tissue on the inner room. After the bottle is placed on the inner developing tissue cultured under room temperature 25 degrees Celsius.2.500 Deluxe for 16 hours per day to observe the experiment
transplantation:The field of plant seedlings grown in the greenhouse crescent of white flowers with peat moss is planting material. Cover the container with a plastic bag cultivation and to control the temperature. After kindergarten for 3 week. Open the plastic cover.
.Recording results:? Takahashi, recording the results from the number of parts that amount, number of explant, number of roots, the ยาวรา, and survival in natural
effects and criticism.
1. Morphology of a white flowers
.A white flowers is a dicot. A taproot La master woody, size up to 15 meters. The twigs and shoots are hairy bark smooth pale grey to Da, cracked rough (picture) ใบเดี่ยว 2A leaves are dark green.Like leaves conjoined twins. The leaves light green hairy leaf size width 8 - 10 cm long, 10 - 13 cm (picture 1B) is a bouquet of flowers of the flowers completely. (Complete flower) flower characteristics asymmetric (Irregular flower) or bilateral symmetry Bilateral symmetry.1 petal petals oval white number 5 petals, stamens and pistil 5 one 1. When flowers bloom. 7 - 12 cm in diameter (picture 1A) a pod, long flat 15-20 cm, the light green, the color is dark brown to you. "(picture 2B) seeds, a flat, round, water colour brown in a pod will seed number 10-25 grain diameter 1.0-1.2 cm (picture 2C)
2 tissue culture of white flowers
.From the study of the bleaching with various methods It is found that the method is suitable for serving in bleaching of a piece of white flowers. The survival of plant parts as high a way as follows. The first purifier with chlorox solution at a concentration of 20.The mixture of catch leaves the skin 1-2 drops for 15 minutes. Rinse with bleaching and the 2 purifier with chlorox solution 10 percent for 15 minutes, then wash with water, distilled 3 times. Long time 2 minutes, which makes plant parts have survived to 78.5 percent.
from culture and the eye in a white flowers on semi-solid food recipes with different concentration MS BAP 5 levels is 0 0.5 1.0,,, and 2.5 5.0 mg / L. after culturing for 8 week feeding added BAP no development, you didn't increase. And there is still only one peak peak development of the NAA concentration, and BAP 0.5 1.0 2.5 5.0 mg / L is developing top 3 - 10 shoots. The top with long, less than 0.5 cm (picture 3) and more than 0.5 cm (picture 4) by NAA BAP concentration 5.0 mg / L. The balance that is longer than the 0.5 cm maximum number 5.05 crest, which significantly different with foods that don't add BAP and fill BAP concentration of other experiments. A significant importance, the statistics. The NAA concentration BAP 1.0 mg / L, a number of top with a length of more than 0.5 cm maximum number 3.65 crest, which significantly different food additive. BAP concentration 2.5 mg / L, with a number of 3.43 amounts but
.Significantly different with the NAA concentration in the experiment BAP other significant importance ยิ่งทาง Statistics (tables), which provide 1 experiments as well as tissue culture in many plants, found that BAP.For example, Girila et al.(1999) study of increasing the number of peaks. From the eye side and survival of the อังกาบสีปูน by cultured on MS fill BAP Kinetin and one found that food recipes. MS fill BAP 1 mg / L.Mangkita et al.(2007) study on increasing the number of the buck week (Fagophyrum tataricum Gaerth.) can cause seizures Na peak to peak 25 when cultured on. MS that BAP 20 thermal M
.The experiments also showed that the plant parts some pieces cultured in medium supplemented with BAP concentration 5.0 mg / L. Development of callus with the same (image 5) which is consistent with the experimental work of Angelon et al.(1992) study found that BA BAP can cause seizures and rice callus. From the cultivation of various kinds, such as the beans, Centrosema brasilianum C. Arenarium C. Macrocarpum,,, C. Pascuorum C. Pubescens. And the C. Virginianum and yet another that the callus was able.
การแปล กรุณารอสักครู่..
