Based on a sample of 3 types of latex gloves are gloves with powder, no type of gloves, there kinds of starch and synthetic rubber type gloves, vinyl gloves, each type will have 1 brand is rubber gloves do not contain wheat flour dough brand gloves are brand Sempermed Progloves and synthetic rubber gloves Semperguard brand vinyl type. And student use of sampling method of lottery number directed by writing the names of the 25 students who, for example, that is random and is not put back into another (Simple Random Sampling without Replacement) student sampling 20 people. Study on protein in rubber gloves 3 pair of each type 20 type is not type with rubber gloves, gloves powder brand Sempermed type powder brand Progloves, synthetic rubber type gloves Semperguard brand vinyl in the extraction of protein from the gloves. A comparative study of temperature is used in extraction between 25° C and 37° C 37° C temperature showed that the extraction of protein from the gloves have more than 25° C temperature control and a comparative study of protein before and after wearing gloves. I found that after wearing gloves to extract protein from the gloves before putting more gloves, using a method. Spectrophotometric assay for measuring protein, measured up to absorb light (absorbance) wavelength 280 nm. From a qualitative study of proteins in sodium dodecyl way gloves sulfate – polyacrylamide gel electrophoresis (SDS-PAGE), which is dyed with Coomassie blue in color not found in protein bars, rubber gloves, all 3 types of color sensitivity, because there are low, Coomassie blue could not know the molecular weight of the protein extract.were first used 1 brand type.
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