Freeze-fracture combined TEM (FF-TEM) provides an excellent tool to visualize individual vesicles suspended in an aqueous medium. Fig. 1 shows typical FF-TEM images of the investigated SSL and REV samples.All samples exhibit uniform particles with close to the regular spherical shape. The surface morphology of SSLs prepared by using membranefilters with nominal pore size of 50 nm (SSL 50) and 100 nm (SSL 100) definitively differs from that of the REV sample
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