We chose to use the Disc diffusion method, method for testing performance of inhibiting the growth of bacteria by a process are as follows:E. coli bacteria, and the bacteria tested S. aureus on sterile Petri plate plate by removing the blank disk (diameter 6 ml) Placed in a Petri dish bacteria according to the defined point. The dropper extracts the test down to coarse. Volume 30-microliter plate blank disk onto a sterile afterwards brought to curing temperature 37 degrees Celsius for a period of 18 hours, and then observe the area caused suppression by measuring the diameter of the circle which is an area in which there is no bacterial growth (Clear zone).
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