Isolation and testing anti-fungal oil, pepper
abstract
isolation and testing of antifungal activity against fungi Candida Albicans pepper oil, which causes opportunistic fungal infections in the hospital, a group of diseases. "Candidiasis" is a fungal infection that causes pathological changes in the mucous membrane of more than 90% percent of people with AIDS is "Candidiasis" When studying the conditions of the separation technique Thin Layer Chromatography by Stationary phase is the plate. TLC Silica gel Mobile phase is Hexane: Ethyl acetate, Hexane: Acetone (3: 7) Ethanol: Hexane (1: 1) and Dichloromethane: Hexane (1: 4) Mobile phase that can extract, black pepper was most obvious Hexane. : Ethyl acetate (1: 1) to see under UV light can result as well. When examined by Agar well diffusion technique is microdilution undilute, 1: 2, 1: 4, 1: 8 and 1:16 to clear the area of fungi in the range 20 ± 0.0 mm., 20 ± 0.0. mm., 15 ± 0.0 mm., 12 ± 0.0 mm and 9 ± 0.0 mm, respectively, conducted by Agar well diffusion using extracts isolated from the TLC plate found a spot on the first and second preferences. Clear the area of fungi at 13 ± 0.0 mm. and 5 ± 0.0 mm, respectively. it is concluded that black pepper extract has the effect of antifungal Candida Albicans depending on the concentration.
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