Colchicine is toxic to other microorganisms. In the first step of adapting the amount Colchicine 0.1 g / l, which Colchicine is a substrate that is emitted by microorganisms after 24 h of incubation, the thiocolchicine. Is slightly soluble in water, ethyl alcohol, 70% used in the preparation thiocolchicine. And mixed in the inoculum. In the inoculum to the following conditions. Microorganisms that have been cultured for more than 10 versions of the rate of growth is limited and under control. At the end of each generation of micro-organisms that have been examined the ability of biotransformation of colchicines and thiocolchicine to a 3-demethyl and glycosyl using TLC and HPLC, the above continues to decrease. colchicine gradually increasing Until a maximum of 10 g / l.
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