Eachsubfraction (7.5 mL) collected

Each
subfraction (7.5 mL) collected was checked for its composition by
TLC using EtOAc
AcOH
H2O (85:10:10, v/v/v) for development,
and dipping in vanillin
sulfuric acid was used in the detection of
compounds with similar skeletons. All fractions were combined into
three portions, I
III. Portion II (fr. 11
14) was purified by HPLC on a
semipreparative reversed-phase column (Hyperprep HSC18 Thermo
Quest, 10250mm, Bellefonte,CA,USA) withMeOH
H2O(7:3, v/v)
as eluent, 2 mL/min, giving 3 (11.8 mg, tR = 11.18 min), 5 (7.3 mg, tR =
23.80 min), and 4 (5.8 mg, tR = 29.60 min). Portion III (fr. 15
18) was
purified by HPLC on a semipreparative reversed-phase column (Biosil
PRO-ODS-U, 10250 mm, Biotic Chemical Co., Taipei, Taiwan) with
MeCN
H2O(58:42, v/v) as eluent, 2 mL/min, affording 1 (6.3mg, tR =
14.98 min) and 2 (3.5 mg, tR = 8.55 min).

ผลลัพธ์ (อังกฤษ) 2:

Each
subfraction (7.5 mL) was checked for its composition Collected by
TLC using EtOAc? AcOH? H2O (85:10:10, v / v / v) for Development,
and dipping in vanillin? Sulfuric acid was used in the detection of
compounds. with similar skeletons. All fractions were combined Into
Three Portions, I? III. Portion II (FR. 11? 14) was Purified by HPLC on a
Semipreparative reversed-Phase column (Hyperprep HSC18 Thermo
Quest, 10? 250mm, Bellefonte, CA, USA) WithMeOH? H2O (7: 3, v / v)
as eluent. , 2 mL / min, giving 3 (11.8 mg, tR = 11.18 min), 5 (7.3 mg, tR =
23.80 min), and 4 (5.8 mg, tR = 29.60 min). Portion III (FR. 15? 18) was
Purified by HPLC on a reversed-Phase Semipreparative column (Biosil
PRO-ODS-U, 10? 250 mm, Biotic Chemical Co., Taipei, Taiwan) with
MeCN? H2O (58:42. , v / v) as eluent, 2 mL / min, affording 1 (6.3mg, tR =
14.98 min) and 2 (3.5 mg, tR = 8:55 min).

ผลลัพธ์ (อังกฤษ) 3:

Each
subfraction (7.5 mL) collected was checked for its composition by
TLC using EtOAc
AcOH
H2O (85: 10: 10 V /, V / V), for development
and. Dipping in vanillin
sulfuric acid was used in the detection of
compounds with similar skeletons. All fractions were combined. Into
three portions I
, III. Portion II (fr. 11
14) was purified by HPLC on a
semipreparative reversed-phase column (Hyperprep. HSC18 Thermo
.Quest 10  250mm,,,, Bellefonte CA USA) withMeOH
H2O (7: 3 V /, V)
as, eluent 2 mL / min giving, 3 (11.8, Mg tR = 11.18 min), 5 (7.3. Mg, the tR
23.80 min), and 4 (5.8, Mg tR = 29.60 min). Portion III (fr. 15
18) was
purified by HPLC on a semipreparative reversed-phase. Column (Biosil
PRO-ODS-U 10 , 250 mm Biotic Chemical, Co, Taipei Taiwan), with
MeCN
H2O (58: 42 V / V), as eluent 2 mL / min,,, Affording, 1 (6.3mgTR
14.98 min) and 2 (3.5, Mg tR = 8.55 min).