Standard graph obtained from HPLC to show the value of each type of substance, its time will vary slightly in each graph and area (concentration) will change to reflect the outcome of the trial.
The graph shows the HPLC standard derived from the value of the substance at that time there will be little change in the graph. And (concentrations) will vary according to the results obtained.
Standard graph obtained from HPLC displays of each type at a time by a slight change in each graph. And space (concentration) is changed according to the experimental results.