The antibacterial properties of the film were tested by Otoni et al. (2557). Escherichia coli and Staphylococcus aureus were inoculated in agar medium, and the culture medium was cultivated in 0 media. 2ml, containing 106 cfu/ml for 24 hours. Then, the PPF film was placed on the culture medium and the membrane-free culture medium respectively, and incubated at 37°C for 24 hours. The inhibition zone was determined by Calipa, and the results were recorded.
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