The experiment was carried out to detect the influence of.Treatment duration on the expression of salt tolerance-related.Genes: 1 L of 200 mM NaCl solution was used to irrigate the.Potted wild R. Rugosa in a total of 16 pots. The first sampling.(0 h) was done before the salt, stress treatment then the young.Leaves were collected after treatment for 6 h 12 h 24 h and,,,48, h respectively. Four pots were randomly selected, for samplingAnd the young leaves were randomly collected from each.Plant. To maintain the stability of salt concentration in the.Treatment period and to prevent the influence of water evaporation.On salt concentration 1 L, of 200 mM NaCl solution.Was irrigated every 6 h.
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