The present study aims to investigate the antibacterial activity of leaves of Alstonia scholaris against bacterial pathogens
by disc diffusion method, well method and incorporating the extract in the media before solidifying. The result of disc
diffusion technique showed that fractions of leaf extract had pronounced antibacterial activity against Methicillin
Resistant Staphylococcus aureus (MRSA) and the clinical strain Providence stuartii. Antibacterial activity was also
tested against a large group of Gram positive and Gram negative bacteria and it was found to reside maximum in the
butanol and ethyl acetate fractions of methanol extract of leaf and bark.