Total polyphenolic content of the p

Total polyphenolic content of the plant extracts was estimated
using microplate assay method modified from standard procedure
using Folin-Ciocalteu reagent [19]. 20 ml each of the 10 mg/ml
extracts in methanol and the serial gallic standard solutions in
methanol were loaded on a 96 well microplate. Then 100 ml of
Folin-Ciocalteu reagent was added to each well, mixed well and
waited for 5 min. Again 80 ml of 7.5% sodium carbonate solution
was added and mixed well. After covering and keeping it in the
dark for 2 hrs, absorbance at 750 nm was measured using Tecan
Infinite M 200 microplate reader. Total polyphenols were
determined using the standard curve obtained for gallic acid.
The estimation of the phenolic compounds in each of the extract
was performed in triplicate and the results were expressed as mg of
gallic acid equivalents (GAE) per g of dry plant part.

ผลลัพธ์ (อังกฤษ) 2:

The total polyphenolic content of Plant extracts was Estimated
Method using microplate assay modified from standard Procedure
using Folin-Ciocalteu Reagent [19]. The 20 ml each of 10 mg / ml
in methanol extracts and The Gallic standard Serial Solutions in
methanol were loaded on a 96 Well microplate. Then 100 ml of
Folin-Ciocalteu Reagent was added to each Well, Well mixed and
waited for 5 min. Again 80 ml ​​of 7.5% Sodium carbonate Solution
was added and mixed Well. After COVERING and Keeping it in The
Dark for 2 hrs, absorbance was measured at 750 NM using Tecan
Infinite M 200 microplate Reader. Total polyphenols were
obtained Determined using Curve The standard for Gallic acid.
The estimation of The Phenolic compounds in each of The Extract
was performed and Triplicate The results were expressed in mg As of
Gallic acid equivalents (GAE) Per Part G of Dry Plant.