Spread plate process 1. the name, d.SC. Students. The trial date at the bottom of the Petri dish.2. pipette 0.1 ml of sample volume-down Petri dish in the middle position.3. dip the l shaped glass rods (spreader) in alcohol, 95 percent concentration, and then tilt the spreader at the edge of the beaker, to extract the excess alcohol.4. glass rods through the alcoholic gradation spreader, dip, burn until the alcohol burns and let cool spreader.5. bring fuel across the plate plate spreader mediation Petri, and be careful not to touch the tool to the edge of the Petri dish.6. dip the spreader in the 95 percent concentration of alcohol and alcohol excess by giving exposure to glass rods of beaker. Leading the flames until the alcohol burns all let cool and put into a dish of bacteria in the mediation Petri dish with the remaining. Step by step the Spread plate shown in Figure 1-4.7. return the dish, Petri, Petri dishes on top, then curing at a temperature of 37 degrees Celsius. 24-48 hours.8. notice the appearance of the colony appearance
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