The first concentration of protein is soluble in ionic strength saline because normal value remains low. Called "salting in". But when the salt concentration increases up the ionic strength, high protein, NaCl is soluble in less and less until the capture of proteins. Settling down Called "salting out".Which protein precipitating out during first intensity to be high MW (0-30%). by the extreme concentration of ammonium sulphate, the more you will make, because it reduced the protein solubility MW in NaCl are small hard to combine the.
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