Method 1 method called analysis of whether the DPPH assay. Assay method of DPPH analysis capability as an antioxidant chemical oxidation (antioxidant), which uses a reagent is 2-diphenyl-1-picrylhydrazyl 2, get the short name of the substance that the DPPH method fast. Easy, accurate analysis and high precision.The principle of theDPPH is a substance that is radical (radical) with stability. (Stable) in each mail type TA Al-Hall pattern final (methanol), this solution is purple, which absorb light very wavelength 515-517 nm (nm)How to react to a radical DPPH • when substances have anti-bacterial or destroy the free radicals. The color of the solution changes to yellow, purple, by comparison with antioxidants, compounds that are used as a standard example is the BHT, if it is capable of destroying free radicals. The intensity of the solution is decreased, or purple radical DPPH decrease quantity. We can measure the intensity of light by Photometer measuring. We do not measure the substance directly, but indirect measurement DHHP is the intensity of the color changes.
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