4.) aqueous solution in an apparatus in a Vortex machine 3 shake for 10 seconds and 60 minutes. Bring the solution has absorption measurements compared to the blank (for each pattern making ethyl acetate)
4) In the third solution was shaken in a Vortex. apparatus for 10 seconds and set aside 60 minutes a solution that can measure absorbance against a black eye. (Solvent ethyl acetate).
4) the solution in an 3 to shake in the machine Vortex apparatus for 10 seconds, and set up the left 60 minutes. The solvent that measured the absorption compared with blank Solvent (ethyl acetate).