What is digital PCR?Digital PCR is

What is digital PCR?

Digital PCR is a technique where the sample containing template DNA is diluted to the point such that aliquoting the diluted sample into multiple reaction chambers (wells/tubes/etc) results in 50% or more of the chambers not receiving the template DNA of interest (figure 1)(see ORIGINS in reference section below). For instance, setting up PCR reactions in a 96-well plate. Using sheared genomic DNA, each well gets 0,1, or more potential DNA fragments. Each fragment represents a single gene loci (red, blue, green, black).

ผลลัพธ์ (อังกฤษ) 1:

what is digital pcr?

digital pcr is a technique where the sample containing template dna is diluted to the point such that aliquoting the diluted sample into multiple reaction chambers (wells / tubes / etc) results in 50% or more of the chambers not. receiving the template dna of interest (figure 1) (see origins in reference section below). for instance, setting up pcr reactions in a 96-well plate.

ผลลัพธ์ (อังกฤษ) 3:

Digital PCR What is?

Digital PCR is a technique where the sample containing template DNA is diluted to the point that such aliquoting the diluted sample into multiple reaction chambers (wells/tubes/etc) results in 50% or more of the chambers not receiving the DNA template of interest (figure 1) (see ORIGINS in reference section below). For instance, setting up PCR reactions in a 96-well plate.sheared genomic Using DNA, each well gets 0, 1, or more potential DNA fragments. Each fragment represents a single gene loci (red, blue, green, black).