HaCaT cells are the immortalized hu

HaCaT cells are the immortalized human keratinocytes and have been extensively used to study the epidermal homeostasis and its pathophysiology. T helper cells play a role in various chronic dermatological conditions and they can affect skin barrier homeostasis. To evaluate whether HaCaT cells can be used as a model cell system to study abnormal skin barrier development in various dermatologic diseases, we analyzed the gene expression profile of epidermal differentiation markers of HaCaT cells in response to major T helper (Th) cell cytokines, such as IFNγ, IL-4, IL-17A and IL-22. The gene transcriptional profile of cornified envelope-associated proteins, such as filaggrin, loricrin, involucrin and keratin 10 (KRT10), in HaCaT cells was generally different from that in normal human keratinocytes (NHKs).

ผลลัพธ์ (อังกฤษ) 2:

HaCaT细胞是永生化的人类角质形成细胞，已被广泛用于研究表皮稳态及其病理生理学。辅助T细胞在各种慢性皮肤病中发挥作用，它们可以影响皮肤屏障的稳态。为了评估HaCaT细胞是否可以用作研究各种皮肤病中异常皮肤屏障发育的模型细胞系统，我们分析了HaCaT的表皮分化标志物对主要辅助T细胞因子（如IFNγ、IL-4、IL-17A和IL-22）的反应基因表达谱。角质化包膜相关蛋白，如聚丝蛋白、洛菌灵、总合蛋白和角蛋白10（KRT10）在HaCaT细胞中的基因转录谱通常与正常人角质形成细胞（NHKs）不同。

ผลลัพธ์ (อังกฤษ) 3:

HaCaT cells are immortalized human keratinocytes, which have been widely used to study epidermal homeostasis and its pathophysiology. T helper cells play a role in various chronic skin diseases and can affect the balance of skin barrier in vivo. In order to evaluate whether HaCaT cells can be used as a model cell system to study the development of abnormal skin barrier in various skin diseases, we analyzed the gene expression profiles of the epidermal differentiation markers of HaCaT cells in response to major T helper (Th) cytokines, su as IFNγ, IL-4, IL-17A and IL-22. The gene transcription profiles of corneal associated protein, such as filaggrin, loricrin, inclusion protein and keratin 10(KRT10) in HaCaT cells are basically different from those in normal human keratinocytes (NHKs).