Given that the sludge was treated with a two-stage anaerobic digestion process at medium temperature in gSTP and then dehydrated prior to farmland application, the majority of the microfibers present in the sludge might be decomposed by microorganisms. As a result, these broken particles were too small to detect using the present method; a similar result was also reported. The crucial factors dictating the microfiber distribution between wastewater and sludge should be investigated further. PP, PE, and PAs were the predominant polymers detected in sludge samples, accounting for more than 60% of both pellets and microbeads which contained a total of 15 types of polymers. Microbeads were made up of PE, PP, and PAs, which accounted for 70% of the total microbeads in the samples. Microfibers were composed of only three polymers (rayon, cellophane, and PET) and 50% of the microfibers were composed of rayon. Interestingly, poly(11-bromoundecyl methacrylate) (PBMA) and PBA were the dominant components of films (53.65%) and fragments (44.44%) (Fig. S3 (A)-(D)).
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