Lyophilized A. vera extract (Coral

Lyophilized A. vera extract (Coral Vegetable, Miyakojima, Japan) and methanolic extract of C. comosum were dissolved to a concentration of 0.1 mol/L in dimethyl sulfoxide (DMSO) as a stock solution and stored at -20 ÀC. The working concentrations used in this study were from 10 ømol/L to 2 000 ømol/L and were freshly diluted with medium before each experiment with a final DMSO concentration of less than 0.1%. Then the extracts were diluted with DulbeccoÊs modified EagleÊs medium to desired concentrations of A. vera (2.50, 7.50 and 10.45 øg/mL) and C. comosum (2.5, 7.5 and 9.6 øg/mL) for 48 h, and control with 1% DMSO. Cells were washed with phosphate buffer saline (PBS) and trypsinized for collecting different aliquots for studying DNA damage, apoptosis markers and cell cycle by flow cytometry.

ผลลัพธ์ (แอฟริกา) 1:

Gevriesdroogde A. vera uittreksel (Coral Groente, Miyakojima, Japan) en methanolic uittreksel van C. Comosum is ontbind om 'n konsentrasie van 0,1 mol / L in dimetiel sulfoxide (DMSO) as 'n voorraad-oplossing en gestoor by -20 AC. Die werking konsentrasies wat in hierdie studie was van 10 ømol / L tot 2 000 ømol / L en was vars verdun met medium voor elke eksperiment met 'n finale DMSO konsentrasie van minder as 0,1%. Dan is die uittreksels verdun met DulbeccoÊs verander EagleÊs medium tot verlangde konsentrasies van A. vera (2.50, 7.50 en 10.45 OG / ml) en C. Comosum (2.5, 7.5 en 9.6 OG / ml) vir 48 h, en beheer met 1%. DMSO. Selle is gewas met fosfaat buffer sout (PBS) en trypsinized vir die invordering van verskillende aliquots vir die bestudering van DNA-skade, apoptose merkers en selsiklus deur vloeisitometrie.