Okwumabua et al. (2003) developed a PCR method that targeted GDH encoding, glutamate dehydrogenase designated GDH PCR.? Marois et al. (2004) also developed a PCR method targeting the 16S, rRNA gene designated 16S rRNA gene PCR. These methods. Have widely been used in clinical practice and laboratories; however these were, designed to detect all 35 serotypes of. Suis S,.Even those that should not have been included (i.e, serotypes 20 22 26,,,,, 32 33 and 34). Therefore a new, PCR assay. Is needed that corresponds to the current reclassification of S. Suis.
The recN gene has been shown to have a lower degree. Of similarity at the species level and higher divergence value at the subspecies level than other housekeeping genes (Glazunova. Et al, 2010).In accordance with, these findings Le et al. (2013) reported that recN sequence analysis revealed complete concordance. With the DNA-DNA reassociation results. These results suggested that the recN gene could be a suitable target for the rapid. Detection of S. Suis.
We here describe a novel PCR method that targets the recN gene of S. Suis designated recN PCR,,And evaluated its potential as an identification system based on a current taxonomical study. I can't speak English well. Sorry, but I need to talk to you everyday.
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