1. weigh 25 g sample into2. pour into the bag at the TSB, there are 225 ml sample.3.Stomacher at medium speed for 1 minute4. filter the sample through a stomacher with a sterile cotton swab, and then.5. bring food samples through filters and then spinning them to hurl at 12000 g * speed for 10 minutes.6. pipette sample into test tubes 9 and 8 ml ml respectively.
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