3.2. Our analysis. Time quantity of Total phenolic
by solvent extraction with solvent, water, dry or 'ton or ethanol. Pipette
ตวั solution. Want, each 20 microliter, put in a test tube design, add water, add 1.58 ML I
.The solution of the Folin-Ciocalteu 100 mixed Ozunu legs, even with dwarf, dead machine Vortex left 5 minutes. Add
sodium carbonate (20% w / V) 300 microliter. Mix well again, and kept in the dark!Room temperature for 2 hours. Then the measured absorbance measurements value
absorb light (Spectrophotometer) (Biochrom Libra S22, S / N 97765 UK,, The 765
.)Nm and find Total Phenolic Compound using gallic acid as standard, the ดบั.
the concentration of gallic acid used to create standard curve is 0 50 100,,, and, 150 200 ppm In
.95% ethanol analysis report in the form of equivalent of Mg gallic acid per 100 mg of water pricing under dry.
.
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