The spot test was performed with the twelve bacteria to assess the
bacterial susceptibility to the bacteriophage (Vieira et al., 2012). The
plates were incubated at 37 °C for 4–12 h. The efficiency of plating
was determined for the bacteria with positive spot tests (occurrence
of a lysis zone) by the double-layer agar method using TSA as culture
medium. Efficacy of plating for each host was calculated by comparison
with an efficacy of 100% for the